Preparation and characterization of a soluble precipitating antigen from African horsesickness virus propagated in cell cultures

The study of African horsesickness virus by the agar double-diffusion precipitation test. Characterization of the precipitation antigen

Characterization and cloning of the African horsesickness virus genome.

The dsRNA profiles of all nine African horsesickness virus (AHSV) serotypes were compared by agarose gel electrophoresis and PAGE. The agarose profiles were identical, but a unique profile was obtained for each of the nine serotypes by PAGE. Nine of the 10 dsRNA genome segments of AHSV-3 were cloned and the clones were used in dot-spot and Northern blot hybridization experiments to determine in...

Preparation of a Dual-Purpose Cell Culture Mumps Virus Antigen for HI and CF Tests

Studies on the replication of African Horse Sickness Virus in two different cell line cultures

Growth of African Horse Sickness Virus in Monkey Kidney Cell Cultures

Detection of bluetongue virus and African horsesickness virus in co-infected cell cultures with NS1 gene probes.

The serogroup specificity of the bluetongue virus (BTV) NS1 and VP3 gene probes was confirmed by means of northern blot hybridization. Under high-stringency conditions both probes hybridized to 22 BTV serotypes (18 South African serotypes, BTV3 from Cyprus and BTV16 from Pakistan) but not to serotypes that originate from Australia and India. Furthermore, NS1 gene probes of BTV and African horse...